actin beta, ACTB
results03

Swissprot ID:

P60709

Protein description:

actin beta, ACTB

Localization in PC12 cells:

actin + neurite tips

A

actin_GFP_loc_NGF_1

A - plasma membrane staining
B - GFP-actin

B

GFP-actin

actin_GFP_loc_NGF_2

merge

actin_GFP_loc_NGF_302

merge:

 

 - plasma membrane

 

 - GFP-actin

Effect of protein overexpression on neurite outgrowth:

no effect on neurite outgrowth

Effect on neurite length:

nontransfected cells

actin_nl_norm

Effect on neurite number:

nontransfected cells

actin_npc_norm

transfected cells

actin_nl

transfected cells

actin_npc

Time lapse of protein dynamics during neurite outgrowth:

 

nontransfected

transfected

average neurite length (microns)

47.9259

47.9157

number of neurites measured

148

164

time

24 hours

 

nontransfected

transfected

average neurite number per cell

2.42623

2.27778

number of cells measured

61

72

time

24 hours

crta203
crta3
actin_1

Time lapse info

technique: GFP
objective: 100x
microscope: Perkin Elmer   Ultraview LCI
image taken every 5 seconds

actin_1.mov
880x724 pixels
size: 22.9 Mb

crta3
crta203
crta203
crta3
actin_3

Time lapse info

technique: GFP
objective: 100x
microscope: Perkin Elmer   Ultraview LCI
image taken every 5 seconds

actin_3.mov
896x684 pixels
size: 10.8 Mb

crta3
crta203