Annexin A4
results03

Swissprot ID:

P09525

Protein description:

Annexin A4

Localization in PC12 cells:

plasma membrane + cytoplasm

A

Annexin-YFP_1

A - plasma membrane staining
B - annexinA4-YFP

B

annexinA4-YFP

Annexin-YFP_2

merge

Annexin-YFP_302

merge:

 

 - plasma membrane

 

 - annexinA4-YFP

Effect of protein overexpression on neurite outgrowth:

no effect on neurite outgrowth

Effect on neurite length:

nontransfected cells

annexin_nl_norm

Effect on neurite number:

nontransfected cells

annexin_npc_norm

transfected cells

annexin_nl

transfected cells

annexin_npc

Time lapse of protein dynamics during neurite outgrowth:

 

nontransfected

transfected

average neurite length (microns)

69.5157

69.957

number of neurites measured

180

197

time

24 hours

 

nontransfected

transfected

average neurite number per cell

1.8

1.60163

number of cells measured

100

123

time

24 hours

crta203
crta3
ON61_position4

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

P09525_a.mov
820x464 pixels
size: 12.1 Mb

crta3
crta203

Comment:

The possible role of annexin A4 in nervous system is described in: Willshaw A, Grant K, Yan J, Rockliffe N, Ambavarapu S, Burdyga G, Varro A, Fukuoka S, Gawler D.: Identification of a novel protein complex containing annexin A4, rabphilin and synaptotagmin., FEBS Lett. 2004 Feb 13;559(1-3):13-21.