Calcium/calmodulin-dependent protein kinase type II beta,CaMK-II beta
results03

Swissprot ID:

Q13554

Protein description:

Calcium/calmodulin-dependent protein kinase type II beta, CaMK-II beta subunit

Localization in PC12 cells:

plasma membrane + cytoplasm

A

CaMKbeta_1

A - plasma membrane staining
B - CaMKbeta-GFP

B

CaMKbeta-GFP

CaMKbeta_2

merge

CaMKbeta_302

merge:

 

 - plasma membrane

 

 - CaMKbeta-GFP

Effect of protein overexpression on neurite outgrowth:

increases neurite length

Effect on neurite length:

nontransfected cells

CaMKbeta_nl_norm

Effect on neurite number:

nontransfected cells

CaMKbeta_npc_norm

transfected cells

CaMKbeta_nl

transfected cells

CaMKbeta_npc

 

nontransfected

transfected

average neurite length (microns)

53.1459

65.8583

number of neurites measured

188

173

time

24 hours

 

nontransfected

transfected

average neurite number per cell

1.91837

1.98851

number of cells measured

98

87

time

24 hours

Comment:

It was shown that CaMKbeta overexpression promotes neurite outgrowth but neurite length measurements were not conducted: Nomura T, Kumatoriya K, Yoshimura Y, Yamauchi T.: Overexpression of alpha and beta isoforms of Ca2+/calmodulin-dependent protein kinase II in neuroblastoma cells -- H-7 promotes neurite outgrowth., Brain Res. 1997 Aug 22;766(1-2):129-41.