cholinergic receptor, muscarinic 2, isoform a (Hypothetical protein)
results03

Swissprot ID:

Q4VBK6

Accession No. / clone ID:

AL832585 / DKFZp451H2216

Protein description:

cholinergic receptor, muscarinic 2, CHRM2, isoform a (Hypothetical protein)

Localization in Vero cells:

plasma membrane

CFP-ORF

ORF-YFP

hlcc1_16h22a
hlcc1_16h22b

Localization in PC12 cells:

plasma membrane

A

2444_loc_4
2444_loc_1a
2444_loc_1

A - plasma membrane staining
B - protein of interest

B

ORF-YFP

2444_loc_5
2444_loc_2a
2444_loc_2

merge

2444_loc_6
2444_loc_3a
2444_loc_3

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

sprouting of numerous small filopodia-like structures inducing similar phentype as overexpression of control protein CDC42.

Time lapse of protein dynamics during neurite outgrowth:

crta203
crta3

AL832585_1
908 x 376 pixels
size: 14.2 Mb

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

crta3
crta203
crta3

AL832585_2
908 x 416 pixels
size: 9.9 Mb

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

crta3
crta203
crta203
crta203
crta3

AL832585_3
908 x 416 pixels
size: 15 Mb

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

crta3
crta203