coiled-coil domain containing 8, CCDC8
results03

Swissprot ID:

Q9H0W5

Accession No. / clone ID:

AL136609 / DKFZp564K0322

Protein description:

coiled-coil domain containing 8, CCDC8

Localization in Vero cells:

plasma membrane

CFP-ORF

ORF-YFP

hfbr2_22k3a

Localization in PC12 cells:

plasma membrane, neurite tips

A

292_loc_NGF_1a

A - plasma membrane staining
B - protein of interest

B

CFP-ORF

292_loc_NGF_2a

merge

292_loc_NGF_3a

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

increases number of neurites per cell

Effect on neurite length:

nontransfected cells

292_nl_norm

Effect on neurite number:

nontransfected cells

292_npc_norm

transfected cells

292_nl

transfected cells

292_npc

Time lapse of protein dynamics during neurite outgrowth:

 

nontransfected

transfected

average neurite length (microns)

57.4618

34.9846

number of neurites measured

138

160

time

24 hours

 

nontransfected

transfected

average neurite number per cell

2.22581

3.72093

number of cells measured

62

43

time

24 hours

crta203
crta3

Q9H0W5.mov
888 x 340 pixels
size: 20.3 Mb

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

crta3
crta203