frizzled homolog 7, FZD7
results03

Swissprot ID:

Q96B74

Accession No. / clone ID:

BC015915 / IMAGE:4549389

Protein description:

frizzled homolog 7, FZD7

Localization in Vero cells:

plasma membrane

CFP-ORF

ORF-YFP

IMAGp958K221240a02

Localization in PC12 cells:

plasma membrane + golgi

A

2027_NGF_102

A - plasma membrane staining
B - protein of interest

B

ORF-YFP

2027_NGF_202

merge

2027_NGF_302

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

increases the number of neurites per cell

Effect on neurite length:

nontransfected cells

2027_length_norm

Effect on neurite number:

nontransfected cells

2027_number_norm

transfected cells

2027_length

transfected cells

2027_number

 

nontransfected

transfected

average neurite length (micron)

39.1732

36.3119

number of neurites measured

168

214

time

24 hours

 

nontransfected

transfected

average neurite number per cell

2.1

2.81579

number of cells measured

80

76

time

24 hours

Time lapse of protein dynamics during neurite outgrowth:

crta203
crta3
2027_1

Q96B74_1.mov
972 x 292 pixels
size: 16.6 Mb

Time lapse info

technique: DIC, GFP
objective: 40x
microscope: LeicaASMDW
image taken every 3 minutes

crta3
crta203
crta203
crta3
2027_4

Q96B74_4.mov
796 x 232 pixels
size: 13.8 Mb

Time lapse info

technique: DIC, GFP
objective: 40x
microscope: LeicaASMDW
image taken every 3 minutes

crta3
crta203