Green Fluorescent Protein (GFP)
results03

Swissprot ID:

 

Protein description:

Green Fluorescent Protein (GFP)

Localization in PC12 cells:

cytoplasm

A

pEGFP_N2_1

A - plasma membrane staining
B - GFP

B

GFP

pEGFP_N2_2

merge

pEGFP_N2_302

merge:

 

 - plasma membrane

 

 - GFP

Effect of protein overexpression on neurite outgrowth:

no effect on neurite outgrowth

Effect on neurite length:

nontransfected cells

GFP_nl_norm

Effect on neurite number:

nontransfected cells

GFP_npc_norm

transfected cells

GFP_nl

transfected cells

GFP_npc

Time lapse of protein dynamics during neurite outgrowth:

 

nontransfected

transfected

average neurite length (microns)

72.4779

70.8946

number of neurites measured

107

103

time

24 hours

 

nontransfected

transfected

average neurite number per cell

1.91071

1.68852

number of cells measured

56

61

time

24 hours

crta203
crta3

gfp.mov
800x548 pixels
size: 7.1 Mb

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

crta3
crta203