GTP-binding protein SAR1 (COPII-associated small GTPase)
results03

Swissprot ID:

Q9NR31

Protein description:

GTP-binding protein SAR1 (COPII-associated small GTPase)

Localization in PC12 cells:

golgi + cytoplasm

A

Sar1_1
Sar1_4

A - plasma membrane staining
B - Sar1-GFP

B

Sar1-GFP

Sar1_2
Sar1_5

merge

Sar1_302
Sar1_6

merge:

 

 - plasma membrane

 

 - Sar1-GFP

Effect of protein overexpression on neurite outgrowth:

no effect on neurite outgrowth

Effect on neurite length:

nontransfected cells

Sar1_nl_norm

Effect on neurite number:

nontransfected cells

Sar1_npc_norm

transfected cells

Sar1_nl

transfected cells

Sar1_npc

 

nontransfected

transfected

average neurite length (microns)

62.0258

55.3361

number of neurites measured

128

121

time

24 hours

 

nontransfected

transfected

average neurite number per cell

2

1.68056

number of cells measured

64

72

time

24 hours