Guanine nucleotide binding protein (G protein) GNAI1
results03

Swissprot ID:

Q9UGA4

Accession No. / clone ID:

AL049933 / DKFZp564K1216

Protein description:

Guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1, GNAI1

Localization in Vero cells:

plasma membrane + golgi

CFP-ORF

ORF-YFP

hfbr2_16k12a
hfbr2_16k12b

Localization in PC12 cells:

plasma membrane + golgi

A

378_loc_NGF_1

A - plasma membrane staining
B - protein of interest

B

ORF-YFP

378_loc_NGF_2

merge

378_loc_NGF_3

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

no effect on neurite outgrowth

Effect on neurite length:

nontransfected cells

378_nl_norm

Effect on neurite number:

nontransfected cells

378_npc_norm

transfected cells

378_nl

transfected cells

378_npc

 

nontransfected

transfected

average neurite length (microns)

61.9562

62.3952

number of neurites measured

179

187

time

24 hours

 

nontransfected

transfected

average neurite number per cell

1.62727

1.57143

number of cells measured

110

119

time

24 hours