Hypothetical protein PRO0971, Gamma-BAR splice variant
results03

Swissprot ID:

Q96GG6

Accession No. / clone ID:

BC009485 / IMAGE:3623656

Protein description:

Hypothetical protein PRO0971, Gamma-BAR splice variant

Localization in Vero cells:

golgi + plasma membrane

CFP-ORF

ORF-YFP

IRALp962K1924a
IRALp962K1924b

Localization in PC12 cells:

golgi + neurite tips

A

1249_loc_NGF_1

A - plasma membrane staining
B - protein of interest

B

ORF-YFP

1249_loc_NGF_2

merge

1249_loc_NGF_3

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

no effect on neurite outgrowth

Effect on neurite length:

nontransfected cells

1249_nl_norm

Effect on neurite number:

nontransfected cells

1249_npc_norm

transfected cells

1249_nl

transfected cells

1249_npc

Time lapse of protein dynamics during neurite outgrowth:

 

nontransfected

transfected

average neurite length (microns)

58.415

58.8732

number of neurites measured

116

128

time

24 hours

 

nontransfected

transfected

average neurite number per cell

1.9661

1.91045

number of cells measured

59

67

time

24 hours

crta203
crta3
427_a03

Time lapse info

technique: DIC, GFP
objective: 40x
microscope: LeicaASMDW
image taken every 3 minutes

Q96GG6_a.mov
560x832 pixels
size: 12.4 Mb

crta3
crta203