Hypothetical protein Q8N3G9
results03

Swissprot ID:

Q8N3G9

Accession No. / clone ID:

AL834349 / DKFZp761H2017

Protein description:

Hypothetical protein

Localization in Vero cells:

golgi

CFP-ORF

ORF-YFP

hamy2_17h20a
hamy2_17h20b

Localization in PC12 cells:

vesicles

A

1790_loc_NGF_1

A - plasma membrane staining
B - protein of interest

B

ORF-YFP

1790_loc_NGF_2

merge

1790_loc_NGF_3

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

no effect on neurite outgrowth

Effect on neurite length:

nontransfected cells

1790_nl_norm

Effect on neurite number:

nontransfected cells

1790_npc_norm

transfected cells

1790_nl

transfected cells

1790_npc

 

nontransfected

transfected

average neurite length (microns)

67.5467

55.8907

number of neurites measured

180

227

time

24 hours

 

nontransfected

transfected

average neurite number per cell

1.875

 1.70677

number of cells measured

96

133

time

24 hours