Hypothetical protein Q8N3J6
results03

Swissprot ID:

Q8N3J6

Accession No. / clone ID:

AL834270 / DKFZp761G128

Protein description:

Hypothetical protein

Localization in Vero cells:

plasma membrane

CFP-ORF

ORF-YFP

hamy2_8g12a
hamy2_8g12b

Localization in PC12 cells:

plasma membrane

A

1102_loc_NGF_1

A - plasma membrane staining
B - protein of interest

B

ORF-YFP

1102_loc_NGF_2

merge

1102_loc_NGF_3

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

no effect on neurite outgrowth

Effect on neurite length:

nontransfected cells

1102_nl_norm

Effect on neurite number:

nontransfected cells

1102_npc_norm

transfected cells

1102_nl

transfected cells

1102_npc

 

nontransfected

transfected

average neurite length (microns)

55.0816

59.4458

number of neurites measured

165

 175

time

24 hours

 

nontransfected

transfected

average neurite number per cell

1.66667

1.49573

number of cells measured

99

117

time

24 hours