Gamma-BAR, AP-1 interacting ptotein
results03

Swissprot ID:

Q9H0V0

Accession No. / cDNA ID:

AL136628 / DKFZp564C182

Protein description:

Gamma-BAR, AP-1 interacting protein

Localization in Vero cells:

golgi + plasma membrane

CFP-ORF

ORF-YFP

hfbr2_2c18a

Localization in PC12 cells:

golgi + neurite tips

A

427_1
427_4

A - DIC
B - protein of interest

B

ORF-GFP

427_2
427_3

Effect of protein overexpression on neurite outgrowth:

30% neurite length reduction

Effect on neurite length:

nontransfected cells

427_nl_norm

Effect on neurite number:

nontransfected cells

427_npc_norm02

transfected cells

427_nl

transfected cells

427_npc02

Time lapse of protein dynamics during neurite outgrowth:

 

nontransfected

transfected

average neurite length (microns)

81.9575

49.6193

number of neurites measured

187

255

time

24 hours

 

nontransfected

transfected

average neurite number per cell

2.10112

1.9842

number of cells measured

89

129

time

24 hours

crta203
crta3

gammaBAR_1.mov
848x364 pixels
size: 51.7 Mb

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: OlympusTILL
image taken every 1 minute

crta3
crta203
crta3
427_b

gammaBAR.mov
724x204 pixels
size: 11.2 Mb

Time lapse info

technique: DIC, GFP
objective: 40x
microscope: LeicaASMDW
image taken every 3 minutes

crta3
crta203
crta203
crta203
crta3

gammaBAR_2.mov
972x788 pixels
size: 17.2 Mb

Time lapse info

technique: GFP
objective: 63x
microscope: OlympusTILL
image taken every 1 minutes

crta3
crta203

Gamma-BAR dynamics in the growth cone

Gamma-BAR dynamics through the stalk

crta203
crta203
crta3
test_2

Q9H0V0_c.mov
648x232 pixels
size: 8 Mb

Time lapse info

objective: 100x
microscope: Perkin Elmer   Ultraview LCI
acquisition: 5 images per second

crta3
crta203
crta3
vl2c18_3

Q9H0V0_d.mov
684x180 pixels
size: 9.4 Mb

Time lapse info

objective: 100x
microscope: Perkin Elmer   Ultraview LCI
acquisition: 5 images per second

crta3
crta203

Gamma-BAR dynamics in the growth cone

crta203
crta3
vl2c18_2

Q9H0V0_e.mov
392x700 pixels
size: 11.2 Mb

Time lapse info

objective: 100x
microscope: Perkin Elmer   Ultraview LCI
acquisition: 5 images per second

crta3
crta203