Hypothetical protein Q9H0V1
results03

Swissprot ID:

Q9H0V1

Accession No. / clone ID:

AL136626 / DKFZp564C012

Protein description:

Hypothetical protein

Localization in Vero cells:

golgi

CFP-ORF

ORF-YFP

hfbr2_2c1a
hfbr2_2c1b

Localization in PC12 cells:

golgi + vesicles

A

1605_loc_NGF_1

A - plasma membrane staining
B - protein of interest

B

CFP-ORF

1605_loc_NGF_2

merge

1605_loc_NGF_3

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

no effect on neurite outgrowth

Effect on neurite length:

nontransfected cells

1605_nl_norm

Effect on neurite number:

nontransfected cells

1605_npc_norm

transfected cells

1605_nl

transfected cells

1605_npc

 

nontransfected

transfected

average neurite length (microns)

62.1408

57.8182

number of neurites measured

147

97

time

24 hours

 

nontransfected

transfected

average neurite number per cell

1.96

1.70175

number of cells measured

75

57

time

24 hours