Hypothetical protein Q9Y4S1
results03

Swissprot ID:

Q9Y4S1

Accession No. :

AL080121

Protein description:

Hypothetical protein

Localization in Vero cells:

golgi/endosomes

CFP-ORF

ORF-YFP

IMAGp958J0886a02

Localization in PC12 cells:

golgi + neurite tips

A

830_NGF_1

A - plasma membrane staining
B - protein of interest

B

ORF-YFP

830_NGF_2

merge

830_NGF_3

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

no effect on neurite outgrowth

Effect on neurite length:

nontransfected cells

830_nl_norm

Effect on neurite number:

nontransfected cells

830_npc_norm

transfected cells

830_nl

transfected cells

830_npc

Time lapse of protein dynamics during neurite outgrowth:

 

nontransfected

transfected

average neurite length (microns)

63.2668

49.3149

number of neurites measured

141

112

time

24 hours

 

nontransfected

transfected

average neurite number per cell

1.78481

1.75

number of cells measured

79

64

time

24 hours

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Q9Y4S1.mov
744x388 pixels
size: 15.7 Mb

Time lapse info

technique: DIC, GFP
objective: 63
microscope: Leica AF6000 LX
image taken every 3 minutes

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