Integral membrane protein 2C, Transmembrane protein BRI3, NPD018
results03

Swissprot ID:

Q9NQX7

Accession No. / clone ID:

AL136603 / DKFZp564L1216

Protein description:

Integral membrane protein 2C, Transmembrane protein BRI3, NPD018

Localization in Vero cells:

plasma membrane + golgi

CFP-ORF

ORF-YFP

hfbr2_16l12a

Localization in PC12 cells:

golgi

A

381_loc_NGF_1

A - plasma membrane staining
B - protein of interest

B

ORF-YFP

381_loc_NGF_2

merge

381_loc_NGF_3

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

no effect on neurite outgrowth

Effect on neurite length:

nontransfected cells

381_nl_norm

Effect on neurite number:

nontransfected cells

381_npc_norm

transfected cells

381_nl

transfected cells

381_npc

 

nontransfected

transfected

average neurite length (microns)

57.8153

60.3811

number of neurites measured

144

214

time

24 hours

 

nontransfected

transfected

average neurite number per cell

2.11765

1.86087

number of cells measured

68

115

time

24 hours