Kinesin-like protein KIF3C
results03

Swissprot ID:

O14782

Protein description:

Kinesin-like protein KIF3C

Localization in PC12 cells:

cytoplasm + neurite tips

A

no NGF

KIF3C_noNGF_1

with NGF

KIF3C_NGF_1

A - plasma membrane staining
B - protein of interest

B

Kif3C-GFP

KIF3C_noNGF_2
KIF3C_NGF_2

merge

KIF3C_noNGF_3
KIF3C_NGF_3

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

no effect on neurite outgrowth

Effect on neurite length:

nontransfected cells

kif3c_nl_norm

Effect on neurite number:

nontransfected cells

kif3c_npc_norm

transfected cells

kif3c_nl

transfected cells

kif3c_npc

Time lapse of protein dynamics during neurite outgrowth:

 

nontransfected

transfected

average neurite length (microns)

53.5789

47.1417

number of neurites measured

194

157

time

24 hours

 

nontransfected

transfected

average neurite number per cell

2.02083

1.8046

number of cells measured

96

87

time

24 hours

crta203
crta203
crta3
ON62_position6

O14782_a.mov
920x444 pixels
size: 14.9 Mb

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

crta3
crta203
crta3

O14782_b.mov
908x329 pixels
size: 19.5 Mb

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

crta3
crta203
crta203
crta3

O14782_c.mov
908x288 pixels
size: 16.8 Mb

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

crta3
crta203