Kinesin family  member 23, KIF23
results03

Swissprot ID:

Q8WVP0

Accession No. / clone ID:

BC017705 / IMAGE:4420248

Protein description:

Kinesin family member 23, KIF23

Localization in Vero cells:

cytoskeleton + microtubules

CFP-ORF

ORF-YFP

IMAGp998G0110157a
IMAGp998G0110157b

Localization in PC12 cells:

microtubules + neurite tips

A

2031_loc_1
2031_loc_4

A - plasma membrane staining
B - protein of interest

B

ORF-YFP

2031_loc_2
2031_loc_5

merge

2031_loc_3
2031_loc_6

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

blocks neurite outgrowth

Time lapse of protein dynamics during neurite outgrowth:

crta203
crta3
Q8WVP0_1

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

Q8WVP0_1.mov
892x392 pixels
size: 9.8 Mb

crta3
crta203
crta203
crta3
Q8WVP0_2

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

Q8WVP0_2.mov
788x364 pixels
size: 11.8 Mb

crta3
crta203

Comment:

blocks neurite outgrowth, induces microtubule bundling, 10% cells are not inhibited and have protein in neurite tips