membrane-associated calcium-independent phospholipase A2 gamma
results03

Swissprot ID:

Q8N3I3

Accession No. / clone ID:

AL834147 / DKFZp761P18121

Protein description:

intracellular membrane-associated calcium-independent phospholipase A2 gamma variant

Localization in Vero cells:

cytoskeleton - actin

CFP-ORF

ORF-YFP

hamy2_121p18a

Localization in PC12 cells:

unknown + neurite tips

A

1808_loc_101103_2

A - plasma membrane staining
B - protein of interest

B

ORF-YFP

1808_loc_101103_1

merge

1808_3

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

no effect on neurite outgrowth

Effect on neurite length:

nontransfected cells

1808_nl_norm

Effect on neurite number:

nontransfected cells

1808_npc_norm

transfected cells

1808_nl

transfected cells

1808_npc

Time lapse of protein dynamics during neurite outgrowth:

 

nontransfected

transfected

average neurite length (microns)

45.097

45.6726

number of neurites measured

156

202

time

24 hours

 

nontransfected

transfected

average neurite number per cell

1.69565

1.4963

number of cells measured

92

135

time

24 hours

crta203
crta3

Q8N3I3_1.mov
912x408 pixels
size: 20 Mb

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

crta3
crta203