Microtubule-associated protein, RP/EB family, member 3
results03

Swissprot ID:

Q9UPY8

Protein description:

Microtubule-associated protein, RP/EB family, member 3

Localization in PC12 cells:

cytockeleton - microtubules

A

B

ORF-GFP

EB3_channels

A - plasma membrane staining
B - protein of interest

Observed phenotypes:

15% +/- 1%

53% +/- 4%

merge

EB3_porcupine

merge:

 

 - plasma membrane

 

 - protein

32% +/- 3%

three_EB3_phenotypes

Effect of protein overexpression on neurite outgrowth:

15% of cells donít respond to NGF, 32% have 4 or more neurites, 53% is not affected by EB3 overexpression.

Time lapse of protein dynamics during neurite outgrowth:

crta203
crta3

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

Q9UPY8_1.mov
436x596 pixels
size: 16.7 Mb

crta3
crta203
crta203
crta3
EB3_4

Time lapse info

objective: 100x
microscope: Perkin Elmer   Ultraview LCI
acquisition: 1 image every 6 seconds

Q9UPY8_4.mov
308x684 pixels
size: 26.1 Mb

crta3
crta203