Microtubule-associated protein, RP/EB family, member 2
results03

Swissprot ID:

Q15555

Accession No. / clone ID:

BC007318 / IMAGE:2989643

Protein description:

Microtubule-associated protein, RP/EB family, member 2

Localization in Vero cells:

cytoskeleton - microtubules

CFP-ORF

ORF-YFP

IRALp962G143a
IRALp962G143b

Localization in PC12 cells:

cytoskeleton - microtubules + cytoplasm

A

2035_102

A - plasma membrane staining
B - protein of interest

Observed phenotypes:

59% +/- 9%

B

ORF-YFP

2035_202

18% +/- 7%

merge

2035_302

merge:

 

 - plasma membrane

 

 - protein

23% +/- 2%

three_EB2_phenotypes

Effect of protein overexpression on neurite outgrowth:

59% of cells donít respond to NGF, 23% have several short, microtubule filled, extensions on the cell surface, 18% is not affected by EB2 overexpression.

Time lapse of protein dynamics during neurite outgrowth:

crta203
crta3
2035_1

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

Q15555_1.mov
864x372 pixels
size: 9.9 Mb

crta3
crta203
crta203
crta3
2035_3

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

Q15555_3.mov
864x264 pixels
size: 8.6 Mb

crta3
crta203
crta203
crta3
2035_2

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

Q15555_2.mov
872x428 pixels
size: 7.6 Mb

crta3
crta203
crta203
crta3
2035_4

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

Q15555_4.mov
856x432 pixels
size: 7.9 Mb

crta3
crta203