Microtubule-associated protein, RP/EB family, member 1
results03

Swissprot ID:

Q15691

Protein description:

Microtubule-associated protein, RP/EB family, member 1

Localization in PC12 cells:

microtubules

A

EB1_loc_NGF_1

A - plasma membrane staining
B - EB1-GFP

B

EB1-GFP

EB1_loc_NGF_2

merge

EB1_loc_NGF_302

merge:

 

 - plasma membrane

 

 - EB1-GFP

Effect of protein overexpression on neurite outgrowth:

no effect on neurite outgrowth

Effect on neurite length:

nontransfected cells

EB1_nl_norm

Effect on neurite number:

nontransfected cells

EB1_npc_norm

transfected cells

EB1_nl

transfected cells

EB1_npc

Time lapse of protein dynamics during neurite outgrowth:

 

nontransfected

transfected

average neurite length (microns)

49.4474

51.2286

number of neurites measured

124

121

time

24 hours

 

nontransfected

transfected

average neurite number per cell

2

2.16071

number of cells measured

62

56

time

24 hours

crta203
crta3

Time lapse info

technique: GFP, DIC
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

EB1_1.mov
904x380 pixels
size: 22.7 Mb

crta3
crta203