PC12 videos
results03
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Time lapse of PC12 cells growing without presence of nerve growth factor (NGF) - proliferation:

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proliferation1

Time lapse info

technique: DIC
objective: 40x
microscope: LeicaASMDW
image taken every 3 minutes

proliferation1.mov
256x296 pixels
size: 5.14 Mb

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proliferation3

Time lapse info

technique: DIC
objective: 40x
microscope: LeicaASMDW
image taken every 3 minutes

proliferation3.mov
320x200 pixels
size: 3.65 Mb

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proliferation2

Time lapse info

technique: DIC
objective: 40x
microscope: LeicaASMDW
image taken every 3 minutes

proliferation2.mov
304x264 pixels
size: 5.76 Mb

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Time lapse of PC12 cells after addition of nerve growth factor (NGF) - differentiation:

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breaking_the_sphere02

Time lapse info

technique: DIC
objective: 40x
microscope: LeicaASMDW
image taken every 3 minutes

breaking the sphere.mov
300x128 pixels
size: 2.1 Mb

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breaking the sphere_b

breaking the sphere_b.mov
560x844 pixels
size: 11.2 Mb

Time lapse info

technique: DIC
objective: 40x
microscope: LeicaASMDW
image taken every 3 minutes

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differentiation1

Time lapse info

technique: DIC
objective: 40x
microscope: LeicaASMDW
image taken every 3 minutes

differentiation 1.mov
312x324 pixels
size: 4.14 Mb

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differentiation3

Time lapse info

technique: FC
objective: 20x
microscope: Till-Photonics TIRF
image taken every 5 minutes

differentiation 3.mov
640x512 pixels
size: 13.3 Mb

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differentiation2

Time lapse info

technique: DIC
objective: 40x
microscope: LeicaASMDW
image taken every 3 minutes

differentiation 2.mov
260x332 pixels
size: 3.52 Mb

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differentiation4

Time lapse info

technique: DIC
objective: 100x
microscope: LeicaASMDW
image taken every 3 minutes

differentiation 4.mov
332x392 pixels
size: 3.63 Mb

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See what happens to time lapse experiments if:

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CO2 is not buffered

blooper1

blooper1.mov
424x372 pixels
size: 5.60 Mb

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humidity is not maintained

blooper2

blooper2.mov
204x172 pixels
size: 1.94 Mb

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the one that happens when you’re recording the most interesting and the most important sequence - focus shift

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blooper3.mov
668x900 pixels
size: 3.6 Mb

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