Neuronal-specific septin 3
results03

Swissprot ID:

Q8N3P3

Accession No. / clone ID:

AL833942 / DKFZp761P16121

Protein description:

Neuronal-specific septin 3

Localization in Vero cells:

cytoskeleton - actin

CFP-ORF

ORF-YFP

hamy2_121p16a

Localization in PC12 cells:

unknown + neurite tips

A

1806_loc_1

A - plasma membrane staining
B - protein of interest

B

ORF-YFP

1806_loc_2

merge

1806_loc_3

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

no effect on neurite outgrowth

Effect on neurite length:

nontransfected cells

1806_nl_norm

Effect on neurite number:

nontransfected cells

1806_npc_norm

transfected cells

1806_nl

transfected cells

1806_npc

Time lapse of protein dynamics during neurite outgrowth:

 

nontransfected

transfected

average neurite length (microns)

45.8622

37.5038

number of neurites measured

191

193

time

24 hours

 

nontransfected

transfected

average neurite number per cell

2.22093

1.89216

number of cells measured

86

102

time

24 hours

crta203
crta3
180603

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

Q8N3P3.mov
798x320 pixels
size: 9.2 Mb

crta3
crta203