Super conserved receptor expressed in brain 2
results03

Swissprot ID:

Q9NPD1

Accession No. / clone ID:

AL161959 / DKFZp761L08121

Protein description:

G protein-coupled receptor GPR85, Super conserved receptor expressed in brain 2

Localization in Vero cells:

plasma membrane + golgi

CFP-ORF

ORF-YFP

hamy2_121l8a
hamy2_121l8b

Localization in PC12 cells:

plasma membrane + golgi + vesicles

A

1505_loc_NGF_2

A - plasma membrane staining
B - protein of interest

B

ORF-YFP

1505_loc_NGF_1

merge

1505_loc_NGF_3

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

no effect on neurite outgrowth

Effect on neurite length:

nontransfected cells

1505_nl_norm

Effect on neurite number:

nontransfected cells

1505_npc_norm

transfected cells

1505_nl

transfected cells

1505_npc

 

nontransfected

transfected

average neurite length (microns)

56.1076

51.3164

number of neurites measured

139

119

time

24 hours

 

nontransfected

transfected

average neurite number per cell

2.13846

1.60811

number of cells measured

65

74

time

24 hours