Epithelial protein lost in neoplasm, EPLIN
results03

Swissprot ID:

Q9UHB6

cDNA ID:

AL136911

Protein description:

Epithelial protein lost in neoplasm, EPLIN

Localization in Vero cells:

cytoskeleton - actin

CFP-ORF

ORF-YFP

hute1_18i19a
hute1_18i19b

Localization in PC12 cells:

actin

A

572_loc_1

A - plasma membrane staining
B - protein of interest

B

ORF-YFP

572_loc_2

merge

572_loc_3

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

no effect on neurite outgrowth

Effect on neurite length:

nontransfected cells

572_nl_norm

Effect on neurite number:

nontransfected cells

572_npc_norm

transfected cells

572_nl

transfected cells

572_npc

 

nontransfected

transfected

average neurite length (microns)

53.7815

53.612

number of neurites measured

185

208

time

24 hours

 

nontransfected

transfected

average neurite number per cell

1.88776

1.6378

number of cells measured

98

127

time

24 hours