Hypothetical protein, DNA polymerase-transactivated protein 6
results03

Swissprot ID:

Q9NTW4

cDNA ID:

AL110124

Protein description:

Hypothetical protein, DNA polymerase-transactivated protein 6

Localization in Vero cells:

cytoskeleton - microtubules

CFP-ORF

ORF-YFP

hfbr2_16a24a
hfbr2_16a24b

Localization in PC12 cells:

microtubules + dots in nucleus

A

777_loc_1

A - plasma membrane staining
B - protein of interest

B

CFP-ORF

777_loc_2

merge

777_loc_3

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

no effect on neurite outgrowth

Effect on neurite length:

nontransfected cells

777_nl_norm02

Effect on neurite number:

nontransfected cells

777_npc_norm

transfected cells

777_nl

transfected cells

777_npc

 

nontransfected

transfected

average neurite length (microns)

55.7458

48.6842

number of neurites measured

116

127

time

24 hours

 

nontransfected

transfected

average neurite number per cell

2

1.69333

number of cells measured

58

75

time

24 hours