Gamma-1-syntrophin, G1SYN, Syntrophin 4
results03

Swissprot ID:

Q9NSN8

cDNA ID:

AL161971

Protein description:

Gamma-1-syntrophin, G1SYN, Syntrophin 4

Localization in Vero cells:

cytoskeleton - filaments

CFP-ORF

ORF-YFP

hamy2_12i23a
hamy2_12i23b

Localization in PC12 cells:

cytoskeleton + cytoplasm + nucleus

A

1509_loc_ngf_1

A - plasma membrane staining
B - protein of interest

B

ORF-YFP

1509_loc_ngf_2

merge

1509_loc_ngf_3

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

no effect on neurite outgrowth

Effect on neurite length:

nontransfected cells

1509_nl_norm

Effect on neurite number:

nontransfected cells

1509_npc_norm

transfected cells

1509_nl

transfected cells

1509_npc

 

nontransfected

transfected

average neurite length (microns)

60.7145

51.3062

number of neurites measured

173

243

time

24 hours

 

nontransfected

transfected

average neurite number per cell

2.1625

2.11304

number of cells measured

80

115

time

24 hours