Hypothetical protein BI828924
results03

Swissprot ID:

 

cDNA ID:

BI828924

Protein description:

Hypothetical protein

Localization in Vero cells:

cytoskeleton - microtubules

CFP-ORF

ORF-YFP

IMAGp998L2111414a
IMAGp998L2111414b

Localization in PC12 cells:

microtubules

A

1564_loc_ngf_1

A - plasma membrane staining
B - protein of interest

B

ORF-YFP

1564_loc_ngf_2

merge

1564_loc_ngf_3

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

no effect on neurite outgrowth

Effect on neurite length:

nontransfected cells

1564_nl_norm

Effect on neurite number:

nontransfected cells

1564_npc_norm

transfected cells

1564_nl

transfected cells

1564_npc

 

nontransfected

transfected

average neurite length (microns)

53.4417

43.8359

number of neurites measured

192

172

time

24 hours

 

nontransfected

transfected

average neurite number per cell

1.90099

1.7732

number of cells measured

101

97

time

24 hours