Hypothetical protein Q9H0W4
results03

Swissprot ID:

Q9H0W4

Accession No. / clone ID:

AL136610 / DKFZp564K0822

Protein description:

Hypothetical protein

Localization in Vero cells:

golgi + endoplasmic reticulum

CFP-ORF

ORF-YFP

hfbr2_22k8a
hfbr2_22k8b

Localization in PC12 cells:

cytoplasm

A

279_loc_NGF_1

A - plasma membrane staining
B - protein of interest

B

CFP-ORF

279_loc_NGF_2

merge

279_loc_NGF_3

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

no effect on neurite outgrowth

Effect on neurite length:

nontransfected cells

279_nl_norm

Effect on neurite number:

nontransfected cells

279_npc_norm

transfected cells

279_nl

transfected cells

279_npc

 

nontransfected

transfected

average neurite length (microns)

68.7979

57.6693

number of neurites measured

121

114

time

24 hours

 

nontransfected

transfected

average neurite number per cell

1.89062

1.54054

number of cells measured

64

74

time

24 hours