proteins used as controls
results03

To determine parameters, reproducibility and sensibility of the assay many control experiment have been needed. This webpage presents the proteins used in control experiments. Proteins are sorted according to their effect on neurite outgrowth in PC12 cells. To look at detailed analysis of protein involvement in neurite outgrowth click on protein thumbnail image or “Protein Name” link. To see the overview of protein’s bioinformatic data click on the “Swissprot” link. Video icon means that time-lapse video of protein dynamics during neurite outgrowth is available.

Proteins with no effect on the neurite outgrowth

A - GFP alone

 

Protein Name

SwissprotID

video

pEGFP_N2_3

Green Fluorescent Protein (GFP)

 

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B - major cytoskeletal components

 

Protein Name

SwissprotID

video

tubulin_YFP_loc_NGF_3

Tubulin alpha 2 , TUBA2

Q13748

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actin_GFP_loc_NGF_3

actin beta, ACTB

P60709

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EB1_loc_NGF_3

Microtubule-associated protein, RP/EB family, member 1

Q15691

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C - major membrane traffic components

 

Protein Name

SwissprotID

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Sar1_3

GTP-binding protein SAR1 (COPII-associated small GTPase)

Q9NR31

 

48202

Ras-related protein Rab-6C

Q9H0N0

 

97002

Ras-related protein Rab-33B

Q9H082

 

161102 

Ras-related protein Rab-1B

Q9H0U4

 

118002

Coatomer beta subunit

P53618

 

D - proteins with clear role in neuronal differentiation and function but not involved in the neurite outgrowth

 

Protein Name

SwissprotID

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113002

Synaptotagmin-3, SYT3

Q9BQG1

 

187603

Synaptoporin

Q8TBG9

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Annexin-YFP_3

Annexin A4

P09525

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Proteins that change the number of neurites per cell

 

Protein Name

SwissprotID

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snap25_loc_3

Synaptosomal-associated protein 25 (SNAP-25)

P60880

 

CDC42_NGF_3

Cell division cycle 42, CDC42

P60953

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Y-2763203

Y-27632, ROCK inhibitor

 

 

Proteins that change the neurite length

 

Protein Name

SwissprotID

video

GFP-VAMP2_3

Vesicle-associated membrane protein 2 (Synaptobrevin 2), VAMP2

Q9BUC2

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CaMKbeta_3

Calcium/calmodulin-dependent protein kinase type II beta, CaMK-II beta subunit

Q13554

 

Proteins that block the neurite outgrowth

 

Protein Name

SwissprotID

video

RhoA_loc_3

Transforming protein RhoA

P61586

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Sar1_GTP_3

GTP restricted dominant negative mutant of Sar1p GTPase (Sar1pH79G)