SACM1L
results03

Swissprot ID:

Q9NTJ5

Accession No. / cDNA ID:

AL136831 / DKFZp434O1328

Protein description:

SACM1L

Localization in Vero cells:

golgi

CFP-ORF

ORF-YFP

htes3_28o13a
htes3_28o13b

Localization in PC12 cells:

golgi

A

no NGF

1660_noNGF_1

with NGF

1660_NGF_1

A - plasma membrane staining
B - protein of interest

B

ORF-YFP

1660_noNGF_2
1660_NGF_2

merge

1660_noNGF_3
1660_NGF_3

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

25% neurite length increase

Effect on neurite length:

nontransfected cells

1660_nl_norm

Effect on neurite number:

nontransfected cells

1660_npc_norm

transfected cells

1660_nl

transfected cells

1660_npc

Time lapse of protein dynamics during neurite outgrowth:

 

nontransfected

transfected

average neurite length (microns)

61.08

73.3391

number of neurites measured

156

164

time

24 hours

 

nontransfected

transfected

average neurite number per cell

1.73333

1.65657

number of cells measured

90

99

time

24 hours

crta203
crta3
1660_1

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

Q9NTJ5_1.mov
836 x 528 pixels
size: 7.6 Mb

crta3
crta203
crta203
crta3
1660_3

Q9NTJ5_3.mov
932 x 308 pixels
size: 6.8 Mb

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

crta3
crta203