Synaptopodin-2, SYP-2
results03

Swissprot ID:

Q9UMS6

Accession No. / clone ID:

AL833547 / DKFZp686M023

Protein description:

Synaptopodin-2, SYP-2

Localization in Vero cells:

cytoskeleton - actin

CFP-ORF

ORF-YFP

hlcc3_3m2a

Localization in PC12 cells:

microtubules

low expression

A

2837_loc_1

high expression

A

2837_102

A - plasma membrane staining
B - protein of interest

B

CFP-ORF

2837_loc_2

B

CFP-ORF

2837_2

merge

2837_loc_3

merge

2837_3

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

blocks neurite outgrowth, 72% of cells have no neurites

Time lapse of protein dynamics during neurite outgrowth:

crta203
crta3
2837_1

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

Q9UMS6_1.mov
992x300 pixels
size: 10.5 Mb

crta3
crta203