Synaptoporin
results03

Swissprot ID:

Q8TBG9

Accession No. / clone ID:

AL834457 / DKFZp547J226

Protein description:

Synaptoporin

Localization in Vero cells:

Golgi + plasma membrane + endoplasmic reticulum

CFP-ORF

ORF-YFP

hfbr1_6j22a
hfbr1_6j22b

Localization in PC12 cells:

Golgi + plasma membrane + neurite tips

A

B

CFP-ORF

1876_1 1876_2

C

A - plasma membrane staining
B - protein of interest
C - blow-up

1876_2a

merge

1876_3

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

no effect on neurite outgrowth

Effect on neurite length:

nontransfected cells

1876_nl_norm

Effect on neurite number:

nontransfected cells

1876_npc_norm

transfected cells

1876_nl

transfected cells

1876_npc

Time lapse of protein dynamics during neurite outgrowth:

 

nontransfected

transfected

average neurite length (microns)

51.0416

54.9672

number of neurites measured

174

191

time

24 hours

 

nontransfected

transfected

average neurite number per cell

1.65714

1.60504

number of cells measured

105

119

time

24 hours

crta203
crta3

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

Q8TBG9.mov
784x398 pixels
size: 8.8 Mb

crta3
crta203