Synaptosomal-associated protein 25 (SNAP-25)
results03

Swissprot ID:

P60880

Protein description:

Synaptosomal-associated protein 25 (SNAP-25)

Localization in PC12 cells:

plasma membrane + golgi

A

snap25_loc_1

A - plasma membrane staining
B - GFP-SNAP25

B

GFP-SNAP25

snap25_loc_2

merge

snap25_loc_302

merge:

 

 - plasma membrane

 

 - GFP-SNAP25

Effect of protein overexpression on neurite outgrowth:

increases number of neurites per cell

Effect on neurite length:

nontransfected cells

snap25_nl_norm

Effect on neurite number:

nontransfected cells

snap25_npc_norm

transfected cells

snap25_nl

transfected cells

snap25_npc

 

nontransfected

transfected

average neurite length (microns)

48.0727

43.8503

number of neurites measured

211

250

time

24 hours

 

nontransfected

transfected

average neurite number per cell

1.93578

2.68817

number of cells measured

109

93

time

24 hours

Comment:

Similar results are obtained by: Shirasu M, Kimura K, Kataoka M, Takahashi M, Okajima S, Kawaguchi S, Hirasawa Y, Ide C, Mizoguchi A.: VAMP-2 promotes neurite elongation and SNAP-25A increases neurite sprouting in PC12 cells., Neurosci Res. 2000 Aug;37(4):265-75.