TBC1 domain family member 3, Rab GTPase-activating protein PRC17
results03

Swissprot ID:

Q8IZP1

Accession No. / cDNA ID:

AL136860 / DKFZp434P2235

Protein description:

TBC1 domain family member 3, Rab GTPase-activating protein PRC17

Localization in Vero cells:

plasma membrane

CFP-ORF

ORF-YFP

htes3_35p22a

Localization in PC12 cells:

plasma membrane

A

1021_NGF_1a03

A - plasma membrane staining
B - protein of interest

B

CFP-ORF

1021_NGF_2a

merge

1021_NGF_3a

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

57% length reduction, high expression toxic

Effect on neurite length:

nontransfected cells

1021_nl_norm

Effect on neurite number:

nontransfected cells

1021_npc_norm

transfected cells

1021_nl

transfected cells

1021_npc

 

nontransfected

transfected

average neurite length (microns)

68.2067

29.5273

number of neurites measured

 128

90

time

24 hours

 

nontransfected

transfected

average neurite number per cell

2.32727

1.47541

number of cells measured

55

61

time

24 hours