Transforming protein RhoA
results03

Swissprot ID:

P61586

Protein description:

Transforming protein RhoA

Localization in PC12 cells:

cytoplasm

A

RhoA_loc_1

A - plasma membrane staining
B - GFP-RhoA

B

GFP-RhoA

RhoA_loc_2

merge

RhoA_loc_302

merge:

 

 - plasma membrane

 

 - GFP-RhoA

Effect of protein overexpression on neurite outgrowth:

blocks neurite outgrowth, 82% of the cells donít develop neurites

Time lapse of protein dynamics during neurite outgrowth:

crta203
crta3
ON66_position4

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

P61586_a.mov
920x508 pixels
size: 17.7 Mb

crta3
crta203
crta203
crta3
ON66_position7

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

P61586_b.mov
908x528 pixels
size: 17.6 Mb

crta3
crta203

Comment:

The role of RhoA in neurite initiation and growth in PC12 cells was described in: Nusser N, Gosmanova E, Zheng Y, Tigyi G.: Nerve growth factor signals through TrkA, phosphatidylinositol 3-kinase, and Rac1 to inactivate RhoA during the initiation of neuronal differentiation of PC12 cells., J Biol Chem. 2002 Sep 27;277(39):35840-6. Epub 2002 Jul 19.