Transmembrane 4 superfamily member 10, TM4SF10
results03

Swissprot ID:

Q9BQJ4

Accession No. / clone ID:

AL136550 / DKFZp761J17121

Protein description:

Transmembrane 4 superfamily member 10, TM4SF10

Localization in Vero cells:

plasma membrane

CFP-ORF

ORF-YFP

hamy2_121j17a

Localization in PC12 cells:

plasma membrane + big vesicles

A

664_loc_NGF_1

A

664_102

A - plasma membrane staining
B - protein of interest

B

ORF-YFP

merge

664_loc_NGF_2 664_loc_NGF_3

B

ORF-YFP

merge

664_202 664_302

merge:

 

 - plasma membrane

 

 - protein

Effect of protein overexpression on neurite outgrowth:

blocks neurite outgrowth, 50% of cells have no neurites, 50% are not affected by overexpression

Effect on neurite length:

nontransfected cells

664_nl_norm

Effect on neurite number:

nontransfected cells

664_npc_norm

transfected cells

664_nl

transfected cells

664_npc

Time lapse of protein dynamics during neurite outgrowth:

 

nontransfected

transfected

average neurite length (microns)

59.6516

64.8531

number of neurites measured

111

113

time

24 hours

 

nontransfected

transfected

average neurite number per cell

1.94737

1.54795

number of cells measured

57

73

time

24 hours

crta203
crta203
crta3
664_1

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

Q9BQJ4_1.mov
832x340 pixels
size: 11.5 Mb

crta3
crta203
crta3
664_2

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

Q9BQJ4_2.mov
1000x484 pixels
size: 10.4 Mb

crta3
crta203
crta203
crta3
664_3

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

Q9BQJ4_3.mov
808x288 pixels
size: 12.1 Mb

crta3
crta203