Tubulin alpha 2 , TUBA2
results03

Swissprot ID:

Q9NRH3

Protein description:

Tubulin alpha 2 , TUBA2

Localization in PC12 cells:

microtubules

A

tubulin_YFP_loc_NGF_1

A - plasma membrane staining
B - tubulin

B

tubulin-YFP

tubulin_YFP_loc_NGF_2

merge

tubulin_YFP_loc_NGF_302

merge:

 

 - plasma membrane

 

 - tubulin

Effect of protein overexpression on neurite outgrowth:

no effect on neurite outgrowth, very high expression blocks neurite outgrowth

Effect on neurite length:

nontransfected cells

tubulin_nl_norm02

Effect on neurite number:

nontransfected cells

tubulin_npc_norm

transfected cells

tubulin_nl

transfected cells

tubulin_npc

Time lapse of protein dynamics during neurite outgrowth:

 

nontransfected

transfected

average neurite length (microns)

53.6078

63.0486

number of neurites measured

121

101

time

24 hours

 

nontransfected

transfected

average neurite number per cell

1.68056

1.29487

number of cells measured

72

78

time

24 hours

crta203
crta3
tubulin_1

Time lapse info

technique: DIC, GFP
objective: 100x
microscope: Leica ASMDW
image taken every minute

tubulin_1.mov
1020x388 pixels
size: 10.9 Mb

crta3
crta203