Vesicle-associated membrane protein 2 (Synaptobrevin 2), VAMP2
results03

Swissprot ID:

Q9BUC2

Protein description:

Vesicle-associated membrane protein 2 (Synaptobrevin 2), VAMP2

Localization in PC12 cells:

golgi + neurite tips

A

GFP-VAMP2_1

A - plasma membrane staining
B - GFP-VAMP2

B

GFP-VAMP2

GFP-VAMP2_2

merge

GFP-VAMP2_302

merge:

 

 - plasma membrane

 

 - GFP-VAMP2

Effect of protein overexpression on neurite outgrowth:

incerases neurite length

Effect on neurite length:

nontransfected cells

vamp2_nl_norm

Effect on neurite number:

nontransfected cells

vamp2_npc_norm

transfected cells

vamp2_nl

transfected cells

vamp2_npc

Time lapse of protein dynamics during neurite outgrowth:

 

nontransfected

transfected

average neurite length (microns)

62.4216

83.4514

number of neurites measured

153

155

time

24 hours

 

nontransfected

transfected

average neurite number per cell

1.77907

1.61458

number of cells measured

86

96

time

24 hours

crta203
crta3
vamp2_1

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

vamp2_1.mov
860 x 900 pixels
size: 16.5 Mb

crta3
crta203
crta203
crta3
vamp2_2

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

vamp2_2.mov
748 x 564 pixels
size: 14.6 Mb

crta3
crta203
crta203
crta3
vamp2_3

vamp2_3.mov
972 x 196 pixels
size: 7.9 Mb

Time lapse info

technique: DIC, GFP
objective: 63x
microscope: LeicaASMDW
image taken every 3 minutes

crta3
crta203

Comment:

Similar results are obtained by: Shirasu M, Kimura K, Kataoka M, Takahashi M, Okajima S, Kawaguchi S, Hirasawa Y, Ide C, Mizoguchi A.: VAMP-2 promotes neurite elongation and SNAP-25A increases neurite sprouting in PC12 cells., Neurosci Res. 2000 Aug;37(4):265-75.