Y-27632, ROCK inhibitor
results03

Swissprot ID:

 

Protein description:

Y-27632, ROCK inhibitor

no NGF
no Y-27632

nothing

no NGF
with Y-27632

Y-2763204

with NGF
no Y-27632

NGF

with NGF
with Y-27632

Y-27632_and_NGF

Effect of Y-27632 treatment on neurite outgrowth:

increases the number of neurites per cell

Effect on neurite length:

+ NGF

NGF_nl

+ Y-27632

Y-27632_nl

+NGF
+ Y-27632

NGF_Y-27632_nl

 

+ NGF

+ Y-27632

+ NGF
+ Y-27632

average neurite length (microns)

58.7357

45.232

62.2402

number of neurites measured

155

189

186

time

24 hours

Effect on neurite number:

+ NGF

NGF_npc

+ Y-27632

Y-27632_npc

+NGF
+ Y-27632

NGF_Y-27632_npc

 

+ NGF

+ Y-27632

+ NGF
+ Y-27632

average number of neurites per cell

2.01299

2.48684

2.65714

number of cells measured

77

76

70

time

24 hours

Comment:

The effect of Y-27632 treatment on hippocampal neurons was shown in: Da Silva JS and Dotti CG: Breaking the neuronal sphere: regulation of the actin cytoskeleton in neuritogenesis., Nat. Rev. Neurosci. 2002 3:694-704.
The role of ROCK in neurite growth has been described in the following article: Hirose M, Ishizaki T, Watanabe N, Uehata M, Kranenburg O, Moolenaar WH, Matsumura F, Maekawa M, Bito H, Narumiya S.: Molecular dissection of the Rho-associated protein kinase (p160ROCK)-regulated neurite remodeling in neuroblastoma N1E-115 cells., J Cell Biol. 1998 Jun 29;141(7):1625-36