Zinc transporter ZnT-7, Solute carrier family 30
results03

Swissprot ID:

Q8NEW0

Accession No. / clone ID:

BX537375 / DKFZp686D18111

Protein description:

Zinc transporter ZnT-7, Solute carrier family 30

Localization in Vero cells:

golgi

CFP-ORF

ORF-YFP

hlcc3_111d18a

Localization in PC12 cells:

golgi + neurite tips

A

2219_loc_1

A - plasma membrane staining
B - protein of interest
C - no NGF

B

CFP-ORF

2219_loc_2

merge:

 

 - plasma membrane

 

 - protein

C

merge

2219_loc_3a
2219_loc_3

Effect of protein overexpression on neurite outgrowth:

no effect on neurite outgrowth

Time lapse of protein dynamics during neurite outgrowth:

crta203
crta3

Q8NEW0_1.mov
778x316 pixels
size: 16 Mb

Time lapse info

technique: DIC, GFP
objective: 63
microscope: Leica AF6000 LX
image taken every 3 minutes

crta3
crta203
crta203
crta3

Q8NEW0_2.mov
956x312 pixels
size: 14.5 Mb

Time lapse info

technique: DIC, GFP
objective: 63
microscope: Leica AF6000 LX
image taken every 3 minutes

crta3
crta203

Comment:

without NGF protein was localised only in golgi and only a few vesicles have been observed. After addition of NGF protein changed the localisation and was predominantly localised in vesicles in neurite tips.